Human LDL receptor gene: HincII polymorphism detected by gene amplification
نویسندگان
چکیده
منابع مشابه
TaqI polymorphism in the human LDL receptor gene.
SOURCE AND DESCRIPTION : The probe that contains exon 1 to 8 was generated from pLDLR3. pLDLR3 contains a full-length LDL receptor cDNA (Yamamoto et at. , 1984) POLYMORPHISM : TaqI identifies a bi-allelic polymorphism with a band of 1.7kb and 0.6kb. FREQUENCY : Studies in 55 unrelated normal Japanese. 1.7Vb fragment 0.69 0.6kb fragment 0.31 NOT POLYMORPHISMS : In 50 individuals no polymorphisms...
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Source and Description: Clone pllH13 has a 2.0 kb insert of F2 cDNA. Chromosomal Localisation: F2 maps to I lpl 1-q12 (Royle et al., 1987). Mendelian Inheritance: Codominant segregation shown in 8 informative families with 21 children. Probe Availability: Freely available from R.T.A. MacGillivray for research purposes. Other Comments: The RFLP variation does not correspond to the allelic varian...
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Southern blot hybridizations with human T cell receptor V beta gene probes were used to determine the sizes of the various V beta gene subfamilies. An analysis of DNA samples from 100 unrelated individuals identified a single individual who lacked one V beta gene segment. A second individual had an apparently different repertoire of V beta gene segments in one subfamily, as assayed by hybridiza...
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Bst EII identifies, a bi-allelic polymorphism in the human LDL receptor gene (l). The 33kb allelc cannot readily be distinguished from the 24kb fragment in the heterozygote using a 0.6 agarose gel. Lower percentage gels often cause handling problems. We suggest co-digestion of DNA with non-polymorphic enzymes such as Eco RI (or Bam HI). Figure 1 shows an autoradiograph of a Southern blot where ...
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Background and Aims: Periodontitis is an inflammatory multifactorial disease in oral tissues and many genetic reasons and environmental factors responsible. Vitamin D deficiency has been determined to be related to periodontal disease. This aim of this study was to investigate the association between rs7975232 polymorphism in vitamin D Receptor gene and periodontitis in 100 patients (as patient...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.14.7215